Fast, Efficient, High-Yield Fueling Biologics R&D

JSBio's Trans CHO Kit Pro includes an upgraded Trans CHO (Basal Medium), and ALLY Feed 100 (Feed Medium). These are completely chemically defined media, free of serum, hydrolysates, and any animal-derived components. The components are synergistically optimized to maximize protein expression yield.

Product Information

Key Features

• Upgraded basal medium Trans CHO: Chemically defined, suitable for various CHO cell subtypes, free of animal-derived components and hydrolysates, ensuring high-quality and high-density suspension culture.
• Feed medium ALLY Feed 100: Used in combination with the basal medium, significantly enhances antibody expression yield and quality.
• Expression enhancer CHO Enhancer: Promotes rapid protein production within a short timeframe.
• High-efficiency transfection reagent Trans 1: Optimized transfection protocol ensures fast and efficient gene integration.

Key Benefits

Case Studies

Trans CHO Kit Pro supports high-efficiency expression of antigens and antibodies. Transfection of CHO-K1 cells was performed at a density of 6 × 10⁶ cells/mL. Experimental results demonstrating the performance of Trans CHO Kit Pro are shown below.

CHO-K1 Cell Transfection Efficiency/Cell Viability Test

Figure 1. Comparison of transient transfection efficiency/cell viability between Trans CHO Kit Pro and PEI transfection in CHO-K1 cells.

Figure 2. Comparison between Trans CHO Kit Pro and the control group (PEI).

Trans CHO Kit Pro demonstrated significantly higher transfection efficiency than the PEI transfection reagent (40 kDa).

Protein Yield

Figure 3. Protein yields comparison for different molecule types between Trans CHO Kit Pro and competitor’s media (control).

When applied to CHO-K1 cells, Trans CHO Kit Pro showed significantly higher yields than that of competitor's media. The yield of Molecule 1 reached 1.4 g/L on day 6.

Comparison of Different Transfection Protocols: Case 1

Figure 4. Comparison of protein yield on day 7, electroporation vs. Trans CHO Kit Pro.

For the same protein molecule and expression time, Trans CHO Kit Pro achieved twice the protein yield comparing to electroporation method.

Comparison of Different Transfection Protocols: Case 2

Figure 5. Comparison of protein yield on day 5 among Competitor's media (control) , Electroporation and Trans CHO Kit Pro.

Transient transfection with Trans CHO Kit Pro consistently showed significantly higher protein yield than both competitor's media (control) and electroporation protocols across different projects. Notably, in Project D, the protein yield reached 2 g/L on day 5.

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