G418是一种氨基糖苷类抗生素 ,在分子遗传试验中，是稳定转染最常用的抗性筛选试剂。它通过抑制转座子Tn601，Tn5 的基因,干扰核糖体功能而阻断蛋白质合成，对原核和真核等细胞产生毒素 ,包括细菌、酵母、植物和哺乳动物细胞 ,也包括原生动物和蠕虫。当neo基因被整合进真核细胞DNA后 ,则能启动neo基因编码的序列转录为mRNA ,从而获得抗性产物氨基糖苷磷酸转移酶的高效表达 ,使细胞获得抗性而能在含有G418的选择性培养基中生长。G418的这一选择特性 ,已在基因转移、基因敲除、抗性筛选以及转基因动物等方面得以广泛应用。
technical data:
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ITEM |
SPECIFICATION |
RESULT |
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Appearance |
White powder |
conforms |
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Solubility (Turbidity) 100mg/mL in H2O |
Clear to Very Slightly Hazy |
conforms |
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Biological Assay |
ED50 Resistant: ≥2500 ug/mL ED50 Sensitive: ≤400 ug/mL |
conforms |
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Absorbance: |
1mg/mL H2O:280nm < 0.015 100mg/Ml H2O: 570nm <0.10 |
conforms |
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pH |
4.6-6.0 |
5.6 |
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Heavy metals |
≤100 ppm |
90 ppm |
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Purity by HPLC
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≥90% |
94.97% |
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** (by Karl Fischer) |
< 10 % |
3.0 % |
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Potency (on a dry basis) |
Not less than 720 ug/mg |
740 ug/mg |
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CONCLUSION: THE RESULTS CONFORM WITH ENTERPRISE STANDARD
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